A PRELIMINARY STUDY ON DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA BY INDIRECT IMMUNOPEROXIDASE ASSAY USING DAUGHTER SPOROCYST-CONTAINING ONCOMELANIA TISSUE SECTIONS
- VernacularTitle:子胞蚴连同钉螺组织切片间接免疫酶染法诊断日本血吸虫病的初步研究
- Author:
Xuandong XIANG
;
Shulong ZHOU
- Publication Type:Journal Article
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Schistosoma japonicum daughter sporocysts in Oncomelania tissue sections were, for the first time, used as antigen in the diagnosis of schistosomiasis japonica by indirect immunoperoxidase assay (IIPA-DS). The most strong peroxidase reaction was localized on the tegument of the daughter sporocysts and cercariae, and at some parts of cercariaf parenchyma, when IIPA-DS was carried out. Of 112 sera from proven cases of schisto-Bomiasis japonica, 106 (94.6%) were positive, the range of titre dilution was from 1:1 to 1:160, the geometrie mean of titres was 1:20. IIPA-DS highly coincided with IHA, COP and ELJSA in both sensitivity and strength of reaction. Sera from 101 blood donors and 24 normal rabbits were all negative. No cross reaction with sera from 24 patients with paragonimiasis was observed.
