A Sensitive,Rapid and Specific Assay for Double Stranded DNA Antibodies
- VernacularTitle:一种敏感、快速、特异的抗双股DNA测定法——短膜虫酶标记抗体技术
- Author:
Yuanwei LEI
- Publication Type:Journal Article
- From:
Chinese Journal of Clinical Laboratory Science
1985;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
This paper presented the method and result of enzyme-immunoassay using the CL kine-toplast as a substrate for the measurement of dsDNA antibodies.Clinical studies showedthat the results of 538 donors,984 non-rheumatic patients and 94 rheumatic diseases otherthan SLE were all negative.The positive rate of anti-dsDNA in 56 SLE sera was 55.4%.The method was compared with indirect fluorescent antibody test(CL-IF).The method isquite sensitive,simple and less expensive and can be performed by the ordinary optical mic-roscope.The micromillimetres of blood from earlobe was satisfactory.
