Urocortin-postconditioning protects heart from ischemia-reperfusion injury through interfering mitochondria-dependent apoptotic pathway
- VernacularTitle:Urocortin后处理抑制线粒体凋亡通路保护心肌缺血再灌注损伤
- Author:
Yuan OU
- Publication Type:Journal Article
- Keywords:
Urocortin;
Myocardial protection;
Apoptosis;
Caspase-3;
CytoC
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Urocortin-postconditioning on mitochondria-dependentapoptotic pathway.Methods: 24 Wistar rats were randomly divided into 3 groups(n=8):Control group(CON),I/R group(I/R),Urocortin group(UCN).The left ventricular samples in the Control group were collected as pre-ischemia controls through thoracotomy.After isolated heart Langendoff and Neely models were established,the rat hearts in the I/R group and the UCN group were continuously perfused with Krebs-Henseleit (K-H)buffersolution for 30 min,then the rat hearts were arrested by cardioplegia,St.Thomas-2 solution(STH-2)for 30min.After that,the rat hearts in the I/R group were reperfused with K-H buffer solution for 90 min.The rat hearts in the UCN group were reperfused with K-H buffer solution containing 10-8 mol/L Urocortin for 90 min.The releasing of Cyto C protein into cytosol was detected by Western blot.The expression of Caspase-3 protein in cardiomyocyte was detected by immunohistochemical assay.The apoptosis index (AI)of cardiomyocyte was detected by TUNEL.Results:Compared with Control group,the expression ofCaspase-3 and Cyto Cprotein in the I/R group and the UCN group were significantly increased(P
