RNAi and Cre-loxP system inhibits GR expression in macrophage RAW264.7 cells of mouse
- VernacularTitle:RNA干扰联合Cre-loxP系统建立小鼠巨噬细胞RAW264.7 GR表达抑制模型
- Author:
Xiaoqing LI
- Publication Type:Journal Article
- Keywords:
GR;
RNAi;
Cre-loxPsystem
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To inhibit GR expression in macrophage RAW264.7 cells of mouse by RNAi and Cre-loxP system.Methods:Two pairs of RNAi target sequence against GR gene were designed and inserted into pBS/U6-Neo vehicle.Recombinant plasmid constructed by Cre-loxP system and RNAi was identified by PCR and sequencing.The recombinant plasmid and Cre-ERT2 plasmid was transfected into the mouse macrophage RAW264.7 cells,in which GR expression was measured on mRNA and protein level respectively by RT-PCR and Western blot.Results:GR expression on mRNA and protein level was significantly inhibited by the two recombinant plasmid against GR gene.Conclusion:Combination using of RNAi and Cre-loxP system could inhabit GR expression in mouse macrophage RAW264.7 cells.
