Effects of MMP7 antisense oligodeoxynucleotide on adhesion and invasion of human lung adenocarcinoma cell A549
- VernacularTitle:MMP7反义寡核苷酸对肺腺癌A549细胞粘附和侵袭能力的影响
- Author:
Bo YANG
- Publication Type:Journal Article
- Keywords:
Matrilysin;
Antisense oligodeoxynucleotide;
Lungadenocarcinoma cell line A549;
Adhesion;
Invasion
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Toinvestigate the influence of MMP7 antisense oligodeoxynucleotide(ASODN)on adhesion and invasion ofhuman lung adenocarcinoma cell line A549.Methods:Phosphorothioate MMP7 ASODN was transfected to A549 cells mediated by liposome. The expression of MMP7 was examined by RT-PCR.The adhesive and invasive ability were examined by plate adhesion model and Boyden Chamber transwell assay.Results:After MMP7 ASODN was transfected,the relative optical density(ROD)of electrophoresis strip was decreased obviously(P
