Expression of osteopontin and its clinico-pathological significance in salivary gland tumors
- VernacularTitle:骨桥蛋白在涎腺肿瘤中的表达及临床意义
- Author:
Minghui ZENG
- Publication Type:Journal Article
- Keywords:
Osteopontin mRNA;
Salivary gland tumors;
Real-time RT-PCR;
Western blot
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To investigate the expression of osteopontin (OPN) mRNA and protein and its correlation with clinico-pathological features of salivary gland tumors. Methods:OPN mRNA and protein level were detected by fluorescent quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR)and Western blot in 41 cases of malignant salivary gland tumors,21 of benign salivary gland tumors and 10 of normal salivary gland tissues. The relationship between the relative content of OPN mRNA and protein and clinico-pathological features of salivary gland tumors were analyzed. Results:The differences of OPN mRNA and protein expression levels in various tissues (malignant salivary gland tumors,benign alivary gland tumors and normal salivary gland tissues) were statistically significant,and the mRNA expression levels were correlated significantly with node metastasis and TNMstage,the expression levels of OPN protein were correlated significantly with node metastasis and TNMstage(P
