Effects of propofol on cell viability and apoptosis in primary cultured cortical neurons of newborn rats
- VernacularTitle:丙泊酚对新生鼠大脑皮层神经元存活及凋亡的影响
- Author:
Kun YANG
- Publication Type:Journal Article
- Keywords:
Propofol;
Cortical neurons;
Cell viability;
Apoptosis;
TUNEL;
Cleaved-caspase3
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of propofol on cell viability and apoptosis in primary cultured cortical neurons of newborn rats.Methods:Primary culture was prepared from newborn rat,and cultured for 6 days.Immunocytochemical and electronic Micoscopic methods were used to identify the cultured neurons.Cells were treated by propofol with different concentrations and at different times.The effects of propofol on neurons were evaluated by cell viability(MTT assay)and markers of apoptosis(TUNEL cell staining and Western-blot of Cleaved-caspase3).Results:There were no significant differences in cell viability among the six groups after 12 h, whereas high concentration of propofol caused an increase in neuronal cell viability after 24 h and 48 h.Propofol incubation for 24 h triggered apoptosis on the neurons(P
