Preliminary study about anti-apoptosis activity after application of the insulin in a rabbit model of corneal alkali burn
- VernacularTitle:胰岛素在兔角膜碱烧伤后抗凋亡作用的初步研究
- Author:
Xia TANG
- Publication Type:Journal Article
- Keywords:
Insulin;
Cornea;
Alkali burn;
Anti-apoptosis
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Toexplore the effectofinsulin(INS) administration on the expression of Bcl-2 protein and the apoptosis of corneal histiocytes following rabbit model of corneal alkali burn.Methods:to establishⅡdegree rabbit model of corneal alkali burn,and respectively to inject normal saline(B group),INS(C group)in the subconjunction each day,and the normal rabbit eyes as controls(A group).The different time after operation the corneal samples were remove and fixed.the apoptosis of corneal histiocytes were detected by light microscope,electron microscope and transferase-mediated dUTP-digoxigenin nick and labeling assay(TUNEL),the expression and alteration of Bcl-2 protein using immunohistochemisty.Results:The TUNEL positive cells were present slightly in the epithelium of normal comea and not present onstroma and endothelium.Bcl-2 were expressed mainly on the normal corneal epithelium,a lower number of Bcl-2 expression on stroma and endothelium.The TUNEL positive cells of group C was stgnificantly lower than that(P
