Optimization of supercritical fluid extraction of paeoniflorin by response surface methodology
- VernacularTitle:响应面法优化白芍中芍药苷的超临界流体提取研究
- Author:
Lei TANG
;
Ben LIU
- Publication Type:Journal Article
- Keywords:
paeoniflorin;
supercritical fluid extraction (SFE);
response surface methodology(RSM);
optimization of extraction condition
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To develop a method for optimizing of supercritical fluid extraction (SFE) of paeoniflorin. METHODS: On the basis of one-factor test and the Box-Behnken central-composite design response surface with 3 factors and 3 levels was adopted. A second order quadratic equation for SFE of paeoniflorin was built. Response surface and contour were graphed with the extraction rate of paeoniflorin as the response value. Based on the analysis of the response surface plots and their corresponding contour plots,the effects of extraction pressure,extraction temperature,and methanol concentration were explored. RESULTS: By using the new method discussed above, the optimum extraction condition was obtained as follows: 18. 9 MPa extraction pressure,extraction cosolvent temperature 59. 9 ℃,68. 5% methanol was chosen as cosolvent with the flow rate of 0. 4 mL/min,and extraction time 60 min. CONCLUSION: Under the optimized condition,extraction rate of paeoniflorin on theoretical value is 7. 85 mg/g.
