Photocytotoxic and apoptosis of hypericin-mediated on nasopharyngeal carcinoma cell line of human in vitro
- VernacularTitle:光诱导金丝桃素对人鼻咽癌细胞CNE-2的毒作用和凋亡研究
- Author:
Xiaoli WANG
;
Caihong WANG
;
Yi GUO
;
Jinlin WANG
;
Junsong ZHANG
- Publication Type:Journal Article
- Keywords:
hypericin;
photodynamic therapy;
light emitting diode;
nasopharyngeal carcinoma;
apoptosis
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study effects of hypericin associated with light emitting diode irradiation on nasopharyngeal carcinoma cell line of human in vitro. METHODS: CNE-2 cells were exposed respectively to different concentration of hypericin,and compared with hematoporphyrin as positive control group,and incubated for 6 h in the dark, then accumulative radiated energe of yellow and red light irradiation equivalent to 5. 67 J/cm2 were given throughout 90 min,killing effect and apoptosis of CNE-2 cells were detected by MTT assay after incubation of 24 h and flow cytometry. RESULTS: MTT assay showed that cytotoxicity of hypericin and hematoporphyrin with light irradiation presented in dose-dependent way,their IC50 values were 0. 049 and 0. 650 ?g/mL,respectively. Flow cytometry showed that hypericin with light irradiation could block cell growth at S phase and G2 phase,when CNE-2 cells were exposed to hypericin at 0. 20 ?g/mL for 18,28 and 48 h,the apoptosis rate reached at 9. 97% ,72. 19% and 92. 24% . CONCLUSION: Hypericin with light irradiation could obviously inhibit the growth of CNE-2 cells and induce apoptosis.
