Simultaneous determination of calycosin and fermononetin in Radix Astragali and its fried product with honey by HPLC
- VernacularTitle:HPLC测定黄芪蜜炙前后毛蕊异黄酮和芒柄花素的含量
- Author:
Shengzhi TIAN
;
Yutao YANG
;
Zhenling ZHANG
;
Liang CHANG
- Publication Type:Journal Article
- Keywords:
Radix Astragali;
Radix Astragali fried with honey;
calycosin;
fermononetin
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To compare the contents of calycosin and fermononetin between Radix Astragali and its fried product with honey. METHODS: The chromatographic column was a Waters SunfireTM C18 column(250 mm ? 4. 6 mm,5 ?m). The mobile phase was the mixture of methanol and water with a linear gradient elution at a flow rate of 1 mL/min. The ultraviolet wavelength was set up 254 nm,and the column temperature was maintained at 35 ℃. RESULTS: The contents of calycosin and fermononetin in Radix Astragali were 0. 247 9 mg/g and 0. 125 2 mg/g respectively,the contents of calycosin and fermononetin in Radix Astragali fried with honey were 0. 225 6 mg/g and 0. 108 2 mg/g,respectively. CONCLUSION: The contents of calycosin and fermononetin in Radix Astragali fried with honey decreased.
