Enzymolysis technology of ginsenoside Rg1 from Panax ginseng by orthogonal design
- VernacularTitle:正交试验优选人参中人参皂苷Rg_1的酶解工艺研究
- Author:
Xiaohu ZHAO
;
Hongmei ZHANG
;
Jinwei YANG
;
Jianpeng GUO
- Publication Type:Journal Article
- Keywords:
Panax ginseng C.A.Mey.;
ginsenoside Rg1;
orthogonal design;
enzymolysis;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the enzymolysis technology of ginsenoside Rg1.Methods Taking ginsenoside Rg1 content as the index,orthogonal design method was used for optimization and HPLC for determination.Results Cellulase enzymolysis was the best extracting process,and enzyme amount,enzymolysis time,and enzymolysis temperature had obvious effect on the extraction of ginsenoside Rg1.The optimum extraction technologies were as follows: cellulase amount was 1.4%,enzymolysis time 60 min,the enzymolysis temperature 45 ℃.Conclusion The optimization extraction technology is simple,steady,and the extracting rate is high.
