Study of inhibitory kinetics of lactamase inhibitors
- VernacularTitle:?-内酰胺酶抑制剂抑酶动力学研究
- Author:
Yongyong LI
- Publication Type:Journal Article
- Keywords:
?-lactamase;
Enzyme kinetic;
?-lactamase inhibitors
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the inhibitory activity of sulbactam,clavulanic acid and tazobactam on the clinical bacterium which produces ?-lactamase.Compare the inhibitory ways and inhibitory effects of three inhibitors.Methods:Study of inhibitory activity of these three ?-lactamase on the 8 bacteria producing ?-lactamase by spectrophotometry.Inhibition constant(K)iwere obtained from dixon plot and the relevance kinetic parameters of inhibition were calculated.Result:All inhibition modality of 3?-lactamase inhibitors are the competitive inhibition.Sulbactam is the weakest of the relative inhibitory effect of three inhibitors,and the strongest is tazobactam. Conclusion:There were differences between inhibitory effects of three enzyme inhibitors on different strains producing ?-lactamase.
