Proteinase-activated receptors regulated the expression of type I/Ⅲ collagen protein of hepatic stellate cell isolated and cultured from SD rats liver
- VernacularTitle:PAR_S调控大鼠HSC合成和分泌胶原蛋白的作用
- Author:
Xiaohong GU
- Publication Type:Journal Article
- Keywords:
Protease activated receptor;
HSC;
Collagen;
Liver fibrosis
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of proteinase-activated receptor 1 and 2(PAR1,PARs)in regulation ofthe expression oftype I/Ⅲ collagen protein of hepatic stellate cell isolated and cultured from SD rats liver.Methods:Hepatic stellate cell isolated and cultured from SD rats livers with a modification technique of Friedman and centrifugation of Nycodenz Gradient.Expression of mRNA for PAR1, PAR2,Ⅰcollagen and Ⅲ collagen were examined by reverse transcription polymerase chain reaction(RT-PCR)and protein examined by immunocytochemistry,observated characteristic cell shape,and examined expression of hexadecanoic acid(HA)and laminin(LN)of supernatant from culturesd HSC.Results:In the course of culturing rat HSC,vitamin A autofluorescence and lipid droplet were decreased gradually or lost following HSC activation,the cells morphology changed to stellate cells and cells proliferation activated.Ⅰ/Ⅲ collagen protein expressions were also increased,the expression of HA,LN,PAR1 and PARs paralleled with the expression ofⅠ/Ⅲ collagen protein and correlated with activation and proliferation of HSC.Conclusion:The expression of PARs correlated with activation and proliferation of HSC,could promote the expression ofⅠ/Ⅲ collagen protein,HA and LN.PARs might play an important role in the mechanisms of hepatic fibrosis.
