Effects of Scutellarin on MUC5AC Mucin Production Induced by Human Neutrophil Elastase or Interleukin 13 on Airway Epithelial Cells.
10.3346/jkms.2011.26.6.778
- Author:
De Peng JIANG
1
;
Juliy M PERELMAN
;
Victor P KOLOSOV
;
Xiang Dong ZHOU
Author Information
1. Department of Respiratory Medicine, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, China. zxd999@263.net
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Scutellarin;
MUC5AC;
Human Neutrophil Elastase;
Interleukin-13;
Airway Epithelial Cells
- MeSH:
Apigenin/chemistry/*pharmacology;
Cells, Cultured;
Dose-Response Relationship, Drug;
Down-Regulation;
Epithelial Cells/*drug effects/metabolism;
Erigeron/chemistry;
Glucuronic Acids/chemistry/*pharmacology;
Humans;
Interleukin-13/*pharmacology;
Leukocyte Elastase/*pharmacology;
Mitogen-Activated Protein Kinase 1/metabolism;
Mitogen-Activated Protein Kinase 3/metabolism;
Mucin 5AC/genetics/*metabolism;
Phosphorylation;
Protein Kinase C/metabolism;
Respiratory Mucosa/drug effects/*metabolism;
STAT6 Transcription Factor/metabolism;
Signal Transduction
- From:Journal of Korean Medical Science
2011;26(6):778-784
- CountryRepublic of Korea
- Language:English
-
Abstract:
Scutellarin is a flavonoid extracted from a traditional Chinese herb, Erigeron breviscapus. The present study investigated the effect of scutellarin on MUC5AC mucin production and the possible mechanism. Human bronchial epithelial 16 (HBE16) cells were pretreated with scutellarin for 60 min, and then exposed to human neutrophil elastase (HNE) or interleukin (IL)-13 for 12 hr. RT-PCR and ELISA were performed to measure the amount of MUC5AC mucin production. The results showed that scutellarin inhibited MUC5AC expression both in mRNA and protein level induced by HNE in a concentration-dependent manner. However, scutellarin failed to inhibit MUC5AC mucin production induced by IL-13. To investigate the intracellular mechanisms associated with the effect of scutellarin on MUC5AC mucin production, western blotting was carried out to examine the phosphorylation of protein kinase C (PKC), signal transducer and activator of transcription 6 (STAT6) and extracellular signal-regulated kinase 1/2 (ERK1/2). The phosphorylation of PKC and ERK1/2 was attenuated after treatment with scutellarin, whereas STAT6 was not significantly affected. Therefore, it is suggested that scutellarin down-regulates MUC5AC mucin production on HBE16 cells via ERK-dependent and PKC-dependent pathways.
