5-Aza-cytidine inhibit proliferation of human promyelocytic leukemia cells (HL60) by inducing apoptosis
- VernacularTitle:甲基化抑制剂5-氮杂胞苷对HL-60细胞增殖及凋亡的影响
- Author:
Yongchun SU
- Publication Type:Journal Article
- Keywords:
Gene methylation;
5-aza-Cytidine;
Leukemia;
Cell cycle;
Apoptosis
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objevtive:This study was aimed to investigate the effect of 5-aza-cytidine on the proliferation,cell cycles distribution profile and apoptosis of human promyelocytic cell line HL60.Methods: The human promyelocytic cell line HL-60 was cultured in RPMI 1640 medium.3-(4,5-Dimethylthiazol-2-y)l-2,5-diphenylte trazolium(MTT)reduction by cells was used to assess drug-induced cell growth inhibition.Cells were treated with 0.25,0.5,1,2.5?mol/L of freshly prepared 5-aza-cytidine for 48 hours before they were harvested for analyzing distribution of cell cycle phases and apoptosis by flow cytometer.Results:5-aza-cytidine inhibited HL60 cell proliferation in a time-and concentration-dependent manner.Apoptosis was induced as detected by flow cytometry.Determination of the cell cycle distribution by flow cytometry revealed an accumulation of cells in G1 phase.Conclusion:DNA methylation inhibitor 5-aza-cytidine has effects on proliferation of human promyelocytic cell line HL60.Possible mechanism of this inhibition is induction of apoptosis.
