Apoptosis of mycosis fungoides hut-102 cells induced by triptolide in combination with dexamethasone and its mechanism
- VernacularTitle:雷公藤内酯醇协同地塞米松诱导蕈样肉芽肿hut-102细胞凋亡及其机制
- Author:
Bo ZHANG
- Publication Type:Journal Article
- Keywords:
Hut-102;
Triptolide;
Dexamethasone;
Glucocorticoid recepto(rGR);
Caspase-3;
Apoptosis
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of triptolide combined with dexamethasone on proliferation and apoptosis of the mycosis fungoides hut-102 cells and the possible mechanisms.Methods:The viability of hut-102 cells was measured by MTT assay;the apoptotic rate of hut-102 cells was detected by flow cytometry.The expression levels of Glucocorticoid receptor(GR)and apoptosis-related protein Caspase-3 were determined by Western blot analysis.Results:①MTT assays showed that different concentrations of triptolide or dexame thasone can effectively inhibit the proliferation of hut-102 cells in a dose and time-dependent manner.When triptolide(12.5 nmol/L)was co-administered with dexamethasone,the inhibition rate increased along with the concentration of dexamethasone.And we observed positive cooperative inhibitory effects of triptolide and dexamethasone on the proliferation of hut-102 cells.②Both triptolide and dexame thasone can independently promote apoptosis of the hut-102 cell.And the apoptotic rate in the combination group was higher than single group(P
