Determination of tetrazanbigen in liver by reversed-phase high performance liquid chromatography
- VernacularTitle:固相萃取RP-HPLC测定小鼠肝脏组织中的德氮吡格
- Author:
Qiang LIU
- Publication Type:Journal Article
- Keywords:
Tetrazanbigen;
Solid phase extraction;
RP-HPLC;
Liver
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a solid phase extraction(SPE)and reversed-phase high-performance liquid chromatographic (RP-HPLC)method for the determination of tetrazanbigen in liver.Methods:Tetrazanbigen in liver samples was determined after a C18 solid phase extraction on a Lichrospher C18 column at 260 nm with an isocratic mobile phase of 90%methanol in 100 ml methanol -deionized water(pH 6.5),containing 0.1%triethyl amine.The flow rate was set at 1.0 ml/min.Results:The linearity was good over the concentration range of 2～20?g/ml(r=0.9 993).The average recovery and RSD were 106.47%and 3.0%respectively.The lower limit of quantification was 1.0?g/ml.Conclusion:The method is simple,accurate and can be used to determine the TNBG concentration in liver and study its metabolism.
