Effect of Different Ratios of Ginsenosides and Panax Notoginsenosides on Proliferation of Rats Peritoneal Mesothelial Cells in Peritoneal Dialysis Solution
- VernacularTitle:人参总皂苷与三七总皂苷不同比例配伍对高糖刺激下腹膜间皮细胞增殖的影响
- Author:
Daixin ZHAO
;
Chuang LI
;
Ling HAN
- Publication Type:Journal Article
- Keywords:
GINSENOSIDES/pharmacology;
PANAX NOTOGINSENOSIDES/pharmacology;
STRENGTHENING QI AND ACTIVATING BLOOD;
PERITONEAL MESOTHELIAL CELLS/pathology;
CELL CULTURE;
PERITONEAL DIALYSIS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different ratios of ginsenosides(GS) and panax notoginsenosides(PNS),which are extracted from Qi-strengthening herbs of Radix Ginseng and blood-activating herbs of Radix Notoginseng respectively,on rats peritoneal mesothelial cells(PMCs) in peritoneal dialysis solution(PDS).Methods PMCs were isolated from rat peritoneal membrane by trypsin digestion method,and then a stable PMCs culture model was established.PMCs were pre-exposed in 4.25% PDS for 3 hours,and then respectively grew in culture solution with different ratios of GS or PNS for 6 hours.The capacity of proliferation of rat PMCs was assessed by methyl thiazolyl tetrazolium(MTT) assay.Results GS 100?g/mL and PNS 80?g/mL showed protective effect on the proliferation of injured PMCs in PDS(P 0.05).Conclusion The combination of GS and PNS,which are extracted from Qi-strengthening herbs of Radix Ginseng and blood-activating herbs of Radix Notoginseng respectively,exerts certain protective effect on PMCs in peritoneal dialysis solution.
