HPLC fingerprint and HPLC-MS analysis for Red Peony Root
- VernacularTitle:赤芍的高效液相指纹图谱及液-质联用分析
- Author:
Yuntao WANG
;
Limei WANG
;
Xiangqun JIN
- Publication Type:Journal Article
- Keywords:
Red Peony Root;
fingerprint;
HPLC;
HPLC-MS
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish an HPLC fingerprint spectrum of Red Peony Root ( Radix paeonia rubra). METHODS:Ten batches of samples were extracted by 50% ethanol and extract liquor was evaporated to dryness,resolved with methanol. The HPLC analysis was performed on a Diamonsil C18 column with the eluting system of gradient acetonitrile and water. MS was applied to the determination of possible chemical structure. RESULTS:Nine main characteristic peaks were selected from the fingerprint spectrum of 10 batches of samples. Five constituents of which were identified as gallic acid,peoniflorin albiflorin,benzoylpaeoniflorin and paeonol based on the MS spectra. CONCLUSION:The method is valuable for quality conthol of Red Peony Root.
