Study on directed differentiation of mesenchymal progenitor cells into neuron-like cells in mice
- VernacularTitle:小鼠间充质祖细胞向神经元样细胞诱导分化的实验研究
- Author:
Li YANG
- Publication Type:Journal Article
- Keywords:
Mesenchymal progenitor cells;
Differentiation;
Neuron-like cells;
Induce
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To culture mesenchymal progenitor cells(MPC)from compact bone fragments in C57 mice in vitro and to study the feasibility of inducing directed differentiation of MPC into neuron-like cells in vitro. Methods:Bones of hind limbs of C57 mice were sheared into bone fragments and digested by collagenase type Ⅱ. Then,MPC were cultured in vitro and analyzed by flow cytometry for identification of its immunology phenotype. MPC of P3 in good growth were induced directionally by the supernatant cultured with primary neuron,and then detected the expression of neuronal specific markers neuron specific enolase(NSE)and neurofilament protein(NF)by immunocytochemical staining. Results:The primary MPC were cultured successfully and they grewwell after passage. Compared with that of the control group,the positive rate of CD29 and CD44 of MPC of antibody group had significant difference (P
