Detection of the immunological competence of HBsAg pulsed Dendritic Vaccination
- VernacularTitle:HBsAg冲击的树突状细胞疫苗的制备及免疫活性研究
- Author:
Shengwei LI
- Publication Type:Journal Article
- Keywords:
Immune depression;
HBV;
Dendritic cell
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the immunological competence of HBsAg-DCs vaccination prepared via HBsAg inducing DCs. Methods:The bone marrow cells of Lewis rat were cultured by RPMI1640 added with 4 ng/ml of granulocyte macrophage colony stimulating factor(GM-CSF)and 8 ng/ml of interlcukin-4(IL-4). 7 days late,the RPMI1640 were added with HBsAg(100 ?g/ml)for another 48h to obtain HBsAg-DCs. The morphologic changes of HBsAg-DCs were observed by light microscope and the phaenotype change were detected by flow cytometry (FCM);The immuno-competence of the HBsAg-DCs were judge by one-way mixed lymphocyte reaction (MLR). Results:There were many DCs growing adherence in cluster and the suspended cells were obviously increasing and much bigger after administrating HBsAg. Compared with DCs,there were significant difference in T cell proliferation stimulated by HBsAg-DCs(P
