The study on gene silence of STAT6 gene by RNA interference
- VernacularTitle:RNA干扰抑制STAT6基因表达的初步研究
- Author:
Dandan LIU
- Publication Type:Journal Article
- Keywords:
STAT6 gene;
STAT6 protein;
RNAi;
shRNA;
Asthma
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct the recombinant plasmid expressing STAT6 shRNA and observe the effects of RNAi suppressed STAT6 gene expression. Methods:shRNA and pIRES2-EGFP-STAT6 plasmid were cotransfected into COS7 cell,to investigate the suppression effect of shRNA on STAT6 under fluorescent microscope . This effect was confirmed by Western blot at protein expression level. Results: STAT6 protein exhibitited high expression in COS7 cells after transfecting pIRES2-EGFP-STAT6 plasmid into COS7 cells alone ; compare with the negative group,the level of the STAT6 protein in the two shRNAs group were 23.1% and 9.6%. Conclusion:STAT6 shRNA were successfully constructed.This technique deserves further study as a potential therapentic tool for asthma .
