Development of fluorescent quantitative RT-PCR method for detection of Hey1 in bone marrow mesenchymal stem cells
- VernacularTitle:骨髓间充质干细胞Hey1基因表达水平荧光定量RT-PCR方法的建立
- Author:
Qiang LIU
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Hey1;
Fluorescent quantitative RT-PCR
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To develop the method of detecting the level ofHey1 expression_the target gene of Notch signaling pathway in bone marrow mesenchymal stem cells,using real-time fluorescence quantitative RT-PCR.Methods:The bone marrow mesenchymal stem cells (C2C12) were cultured and the total RNA was extracted.And then the amplification efficiency was verified between the reference gene ?-actin and the target gene Hey1.To optimize the PCR conditions and analyse amplification curves and melting curve before validation of quantitatve PCR products with gel electrophoresis.Results:The reference gene?-actin and the target gene Hey1 have the same amplification efficiency(slope difference of the amplification curve
