Distribution of transplanted bone mesenchymal stem cells in colon of ulcerative colitis rat
- VernacularTitle:移植骨髓间充质干细胞在溃疡性结肠炎大鼠结肠的分布
- Author:
Xiaoyun CHEN
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Transplantation;
Ulcerative colitis
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the optimal dose of transplanted bone marrow mesenchymal stem cells (BMSCs) for the ulcerative colitis rat. Methods:The BMSCs of SD rat were isolated,cultured and labelled with DAPI.Colitis of rat was induced with immunecombined TNBS/ethanol in group A,B.and C. Each rat in 3 groups received caudal vein injection of fluids containing 1?106,5?106, and 1?107 BMSCs respectively. 5 rats in each group were sacrificed at day 7 and 14 after injection.Cryostat sections of colon were prepareted.The numbers of BMSCs in focal area of colitis and surrounding normal tissue were counted under fluorescent microscope. Results:The DAPI marked BMSCs could been seen in the colic mucosa in each group on day 7 and 14. The number of BMSCs in the ulcerated area was bigger than that in the surrounding normal tissue (P
