Hypertonic saline and pentoxifylline reduces hemorrhagic shock resuscitation-induced pulmonary inflammation through inhibiting the expression of NF-?B
- VernacularTitle:HSPTX通过抑制NF-?B蛋白表达减轻失血性休克复苏诱发的肺部炎症反应
- Author:
Yu LIU
- Publication Type:Journal Article
- Keywords:
Pentoxifylline;
Hypertonic saline;
Hemorrhagic shock;
Acute lung injury;
Tumor necrosis factor-?;
Nuclear factor kappa-B
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of hypertonic saline and pentoxifylline resuscitation on pulmonary inflammation following hemorrhagic shock in rats. Methods:Controlled hemorrhagic shock was induced by blood withdrawal (MAP,40 mmHg)and maintained for 60 min. Animals were randomly divided into 3 groups:① Sham animals(n=8)underwent cannulation without shock or resuscitation and served as negative controls;②RL-resuscitated animals (n=8)received 32 ml/kg RL;③HSPTX group received 4 ml/kg of 7.5% NaCl+25 mg/kg of PTX. PaO2,pH,PaCO2,wet/dry lung weight ratio(W/D),and lung penetrating index were determined. The percentages of neutrophil in bronchoalveolar lavage fluid,the levels of malonadialdehyde (MDA)and superoxide dismutase (SOD)were measured. The tumor necrosis factor-?(TNF-?)and interleukin 1?(IL-1?)in the supernatant of bronchoalveolor lavage fluid were determined by ELISA method,and the expression of NF-?B in the lung tissues was quantified by Western blot. Results:PaO2 and pH were increased whereas PaCO2 was decreased in HSPTX group when compared with that in RL group (P
