Determination of madecassoside in SD rats plasma by solid-phase extraction RP-HPLC
- VernacularTitle:固相萃取-反相高效液相色谱法测定大鼠血浆中羟基积雪草苷的浓度
- Author:
Xinwei TAN
- Publication Type:Journal Article
- Keywords:
Madecassoside;
Solid-phase extraction(SPE);
HPLC
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for madecassoside determination by solid-phase extraction RP-HPLC in SD rats plasma. Methods:The sample was cleaned with an Strata-X solid-phase extraction . HPLC conditions were as follows:Welchrom-C18 column (250 mm?4.6 mm,5 ?m),water-acetonitrile(24∶76)as mobile phase(flowrate:1.0 ml/min),cucurbitacin B as internal standard, detection wavelength of 225 nm,and column temperature of 30℃. Results:In this situation,the remaining times of the madecassoside and cucurbitacin B were 5.11 min and 8.14 min,respectively. The linear range of the method was 5 ?g/ml～1.5 mg/ml (r=0.999 8).The low limit of detection was 1 ?g/ml. The method recoveries and the extraction recoveries were 90.21%～95.65% and 80.65%～87.04%,respectively. The within-day RSD and between-day RSD were between 1.31%～3.07%(n=5). Conclusion:Themethod is simple,convenient,accurate, reproducible,and suitable for studying the pharmacokinetics of madecassoside in SD rats .
