Isolation and antitumor activity in vitro of three kinds of bufadienolides
- VernacularTitle:蟾酥中蟾毒配基类成分的分离纯化及其体外抗肿瘤活性的研究
- Author:
Dan LIU
;
Lin ZHU
;
Jianfang FENG
- Publication Type:Journal Article
- Keywords:
Venenum bufonis;
bufalin;
cinobufagin;
resibufogenin;
separation and purification;
antitumor ac- tivity
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To isolate three kinds of bufadienolides composition from Venenum bufonis in order to investigate their antitumor activity in vitro.METHODS: Alcohol extraction and isolation by Silica gel colume were employed in this study.Their antitumor activities in vitro were evaluated by MTT colorimetric method.RESULTS: With such methods,three kinds of bufadienolides,including Bufalin,cinobufagin,and resibufogenin with purity above 90% were prepared.The in-vitro results showed that three kinds of bufadienolides had prominent inhibition effect on human lung cancer A549 cells,human breast cancer MDA-MB-435 cells in the range of 0.001 ?g/mL ～ 100 ?g/mL.CONCLUSION: Isolation by Silica gel colume can be used to separate three kinds of bufadienolides from Venenum bufonis and its constituents has prominent anti-cancer effects.
