Effect of WIN55,212-2 on the Expression of PGE_2 in Cultured Bovine Trabecular Meshwork Cells
- VernacularTitle:大麻素WIN55,212-2对体外培养牛眼小梁细胞前列腺素E_2表达的影响
- Author:
Wenfeng ZHAO
;
Wanhui WANG
- Publication Type:Journal Article
- Keywords:
WIN55,212-2;
trabecular meshwork cell;
PGE2;
PGE2 mRNA
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of WIN55,212-2 on the expression of PGE2 in cultured bovine trabecular meshwork cells(TMCs) and discuss its mechanism of reducing the intraocular pressure.Methods TMCs were obtained through the cultured tissue,bovine TMCs were prmiarily cultured and subcultured.The third generation bovine TMCs were incubated with different dosages of WIN55,212-2 and the supernatant was collected.The concentration of PGE2 in the medium was measured by ELISA method.PGE2mRNA in TMCs was analyzed by RT-PCR.Result PGE2 in the supernatant and PGE2mRNA in TMCs increased depending on the dosages of WIN55,212-2.Conclusion Certain concentration of WIN55,212-2 may promote PGE2 secretion of TMCs,so as to reduce the intraocular pressure.
