Effects of Qigefang on Cell Proliferation,Movement,Secreting MMP-2 and MMP-9 of MGC Gastric Cancer Cell Line
- VernacularTitle:启膈方对人胃癌MGC细胞增殖、运动能力及分泌MMP-2、MMP-9酶活性的影响
- Author:
Jing LI
;
Na LI
;
Yaxian LIU
- Publication Type:Journal Article
- Keywords:
Qigefang;
cell culture;
cell proliferation;
cell movement;
MMP-2;
MMP-9
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the inhibitory action of Qigefang on cell proliferation,movement,secreting MMP-2 and MMP-9 of MGC gastric cancer cell line.Methods MGC cell line of gastric cancer was cultured and divided into five groups randomly:low-,middle-,high-dose group of Qigefang,5-FU group,control group.Qigefang was added in Qigefang group to make final concentration of 35,70,140 mg/mL,5-FU in chemothapy group to 50 ?g/mL,and PBS in control group.Tumor cell proliferation was detected by MTT,moving ablity of tumor cell by agar drop,and MMP-2,MMP-9 activity by gelatinase zymography.Results Qigefang had obviously depressant effect on proliferation of MGC cell.It became more obviously with raising drug density.Moving ability of all dose Qigefang groups and 5-FU group were more feeble than control group.The difference was significant(P
