Effects of Zuoguiwan and Youguiwan on Immunohistochemical Expression of Lymphocyte Subpopulation in Central Nervous System of EAE Rats
- VernacularTitle:左归丸和右归丸对自身免疫性脑脊髓炎大鼠中枢神经系统淋巴细胞亚群免疫组化表达的影响
- Author:
Yongping FAN
;
Lijun SONG
;
Ming YE
- Publication Type:Journal Article
- Keywords:
experimental autoimmune encephalomyelitis;
Zuoguiwan;
Youguiwan;
multiple sclerosis
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the immunohistochemical expression of lymphocyte subpopulationin in brain and spinal cord tissue,and explore the mechanism of Zuogui wan and Yougui wan on rats with experimental autoimmune encephalomyelitis(EAE).Methods Lewis rats were immunized with the myelin basic protein(MBP).120 rats were grouping randomly into normal group,EAE group,prednisone group,Zuoguiwan group and Youguiwan group after post immunization(PI).Rats in normal group and EAE group were administered normal soline,each 3 mL/d.Rats in prednisone group were administered suspension of prednisone after developed clinical signs,each 5 mg/kg.Rats in Zuoguiwan group were administered suspension of Zuoguiwan,each 2 g/kg,and rats in Youguiwan group were administered suspension of Youguiwan,each 3 g/kg.On 15th and 27th day after PI,rats were killed and the immunohistochemical staining was performed on the sections of brain and spinal cord.Results On 15th and 27th day after PI,the expression of CD4+ in brain and spinal cord tissue of EAE group were significantly higher than that in normal group(P
