Uniform design for in vitro somatic embryogenesis and plant regeneration of Gymnadenia conopsea
- VernacularTitle:基于均匀设计优化手参体细胞胚胎发生及植株再生体系
- Author:
Dizhou GU
;
Ying FENG
;
Meiying GU
;
Yanping WANG
;
Yuntian JIANG
- Publication Type:Journal Article
- Keywords:
Gymnadenia conopsea(L.) R.Br.;
uniform design;
embryogenic cell complex;
somatic embryo;
plant regeneration
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Using stem tip of Gymnadenia conopsea in this experiment to investigate somatic embryogenesis and plant regeneration in vitro.Methods Uniform dedign for the most suitable media for embryogenic callus induction and embryogenic cell complex,development of somatic embryo and plant regeneration were screened.Results The results showed that Chaturvedi and Mitra(CM)+6-BA 1.0 mg/L+IAA 2.0 mg/L+2,4-D 0.1 mg/L was fit for embryogenic callus and embryogenic cell complex induction.Percentage was 98%;The medium of development of somatic embryo and plant regeneration was CM+6-BA 1.00 mg/L+IAA 0.10 mg/L.Percentage was 100% and converted into plantlets with shoots and roots after 50 d culture on the same medium.Conclusion The observation of morphostructure and ultrastructure proves the process of somatic embryogenesis of G.conopsea.
