Studies on chemical components of lyophilized powder of Sinisan
- VernacularTitle:四逆散冻干粉化学成分变化的研究
- Author:
Yuefeng LI
;
Xingke YAN
;
Tingli LI
;
Yuxin HUANG
- Publication Type:Journal Article
- Keywords:
lyophilized powder of Sinisan;
fingerprinting;
varation of chemical components
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The chemical components of lyophilized powder of Sinisan were analyzed by the method of RP-HPLC.Methods HPLC Method was applied for quality assessment on lyophilized powder of Sinisan.Column: Kromasil C18 column(250 mm ? 4.6 mm,5 ?m);wavelength: 210 and 240 nm;flow-rate: 1.0 mL/min;column temperature: 28 ℃.Mobile phase: acetonitrile(A) and H2O(acidified to pH=3 with phosphoric acid)(B).Concentration of lyophilized powder of Sinisan was resolved into different parts and analyzed by the same method,the variation of the chemical components was analyzed.Results At 240 nm,21 peaks were detected and two of them were confirmed to be from Bupleurum chinense,three from Paeonla lactiflora,ten from Citrus aurantium,and five from Glycyrrhiza uralensis.At 210 nm,24 peaks were detected and one of them was confirmed to be from B.chinense,four from P.lactiflora,fourteen from C.aurantium,and five form G.uralensis.Conclusion This method can be used to establish the fingerprint chromatograph of lyophilized powder of Sinisan and elucidate the variation of chemical components completely.
