Establishment of hemolytic anemia rat model and its laboratory evaluation
- VernacularTitle:溶血性贫血大鼠模型的建立及实验评价
- Author:
Bo HUANG
- Publication Type:Journal Article
- Keywords:
Hemolytic anemia;
Rat animal model;
Laboratory evaluation
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Study on establishing HA animal model,which isoperated simply and have short model building period and high successful rate,to provide favorable test materials for HA-related scientific research.Methods:10 SD rats were randomly selected to set up the model by using APH intraperitoneal injection,meanwhile rats intraperitoneally infused with saline served as controls group.After 8 days of experimental treatment,we evaluated the animal model by using blood analysis,reticular count,myelogram and other laboratory experimental results.Results:On eighth day after experimental treat,the results of RBC、HGB、PLT、Ret% in Rats'peripheral blood are respectively(2.98?0.623)?1012/L、(100?14.2)g/L、(320?358.8)?109/L、90.7%?4.23%,the concentration of indirect bilirubin and free-haemoglobin in plasma are respectively(10.3?3.30)?mol/L、(1 248?282.1)mg/L.Compared to the control group,the difference of the results all have the statistical significance;bone marrow exhibited that karyocytes proliferate obviously,mainly the erythroid system.Conclusion:APH intraperitoneal injection can set up HA animal model shortly and conveniently,and the animal model has the clinical features.So the method of APH intraperitoneal injection is an ideal way to establish HA animal model
