Experimental research on anti-free radicals protection of intranasal administration of granulocyte colony stimulating factor following experimental subarachnoid hemorrhage
- VernacularTitle:经鼻给予粒细胞集落刺激因子对蛛网膜下腔出血后抗自由基脑保护作用的实验研究
- Author:
Fenying CHEN
- Publication Type:Journal Article
- Keywords:
Subarachnoid hemorrhage;
Intranasal administration;
Free radicals;
Granulocyte colony stimulating factor
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether intranasal administration of recombinant human granulocyte colony stimulating factor can relieve free radicals injury following experimental subarachnoid hemorrhage.Methods:Rat SAH models was induced by double injection of autologous blood into the cisterna magna.The rats were randomizedly divided into normal control group,SAH group,SAH+NS group,and SAH+rhGCSF group.Coronal sections were made to observe the neuropathology of the cortex and the CA1 region of the hippocampus.Superoxide dismutase(SOD)activity and content of malondialkhyde(MDA)in the brain tissue homogenate were detected 24 hours after secondary cisternal injection.Results:①Compared with normal control group,the number of neuronal cells in cortex and hippocampal CA1 region were significantly reduced,the structural arrangement was disordered and morphology was abnormal in SAH group and SAH + NS group,rhGCSF treatment could relieve this injury.②SOD activity was significantly decreased and the content of MDA was significantly increased on 24 hours after SAH induction.There is no statistically significant difference between SAH group and SAH+NS group.The content of MDA was decreased(P
