Effect of intrathecal down-regulation of 5-HT_(5A) receptors by RNA interference in rats with chemotherapy-evoked hyperalgesia
- VernacularTitle:鞘内靶向沉默5-HT_(5A)受体基因对化疗痛的影响
- Author:
Weixiang MIN
- Publication Type:Journal Article
- Keywords:
5-HT5 Areceptor;
Chemotherapy;
Neuropathic pain;
Intrathecal injection;
RNA interference
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of 5-HT5A receptors in rats with chemo the rapy-evoked hyperalgesia by intra the calinjection of adenovirus-mediated small-interference RNA.Methods:Adult male SD rats were randomly divided into 2 groups:model group(n=10,intraperitoneal injection of vincristine)and control group(n=10,intraperitoneal injection of normal saline).The mechanical hyperalgesia were measured as the paw withdrawal thresholds(PWT)and the expression of 5-HT5AR mRNA in L4、5 spinal cord were determined by RT-PCR.Thirty rats with chemotherapy-evoked hyperalgesia were randomly divided into 3 groups after the formation of chemotherapy pain model:experimental group,negative control group and blank control group,which respectively were treated with intrathecal injection of Ad-5-HT5AR-siRNA,Ad-X-HK and normal saline.Expressions of 5-HT5AR mRNA and PWT after intrathecal injection were assessed respectively.Results:Compared with control group,PWT of rats in model group were significantly decreased(P
