DNA microarray screening and analysis of the virulence gene in golden hamster cheek poch mucosa with precancerous lesions
- VernacularTitle:用全基因芯片技术对金黄地鼠口腔颊黏膜癌前病变相关基因的筛选和分析
- Author:
Guodong ZHANG
- Publication Type:Journal Article
- Keywords:
Cheek poch mucosa;
Precancerous lesions;
Gene expression profiling;
Gene chip
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The aim of this study was to screen and analysize the important virulent genes in golden hamster cheek pochmucosa with precancerous lesions induced by DMBA.Methods:The models of golden hamster cheek poch mucosa precancerous lesions were induced by DMBA(styrene-dimethyl-anthracene).We extracted the total RNA from the normal mucosa and precancerous lesions;then we respectively used the Cy3 to mark the cDNA of normal mucosa and precancerous lesions;and used the Agilent Rat microarray containing 41,000 genes/ESTs to screen the differentially expressed genes;used the screening criteria of Log2Ratio ≥2.5 and Log2Ratio≤-2.5,at last using the Gene Ontology to analysis the biology function of the important virulent genes.Meanwhile,we confirmed the correctness of the results of the Gene Chip by using the RT-PCR to verificate some important pathogenic genes.Results:We detected a total of 1331 differentially expressed genes(up-regulated 747;down-regulated 584).GO analysis showed that these differentially expressed genes mainly related to the macromolecular metabolism,signal transduction and so on.The RT-PCR results of Atp6v0d2 and Sfrp2 were consistent with the gene chip analysis.Conclusion:Towards the screening and analysis of the important differentlily expressed genes in the gold hamster cheek pouch mucosa with precancerous lesions,we found that the special stage-precancerous lesions involved in changes in multiple genes.The further study of these genes is useful to explore the pathogenesis of precancerous lesions in the oral mucosa and effectively prevent the occurrence of precancerous lesions.
