Effect of Ganle Keli on the Expression of ?-Smooth Muscle Actin in Hepatic Fibrosis Rats
- VernacularTitle:肝乐颗粒对肝纤维化大鼠肝组织?-平滑肌肌动蛋白表达的影响
- Author:
Hui JIANG
;
Jiarong GAO
;
Jiafu ZHANG
- Publication Type:Journal Article
- Keywords:
Ganle keli;
liver fibrosis;
?-SMA;
rat
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2008;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the change of ?-smooth muscle actin (?-SMA) expression in carbon tetrachloride (CCl4)-induced liver fibrosis in rats after treatment with Ganle keli. Methods Liver fibrosis rat model was induced by CCl4 twice a week for 12 weeks. Ganle keli was used daily intragastric administration from the 7th week. Contents of NO, NOS and iNOS were determined by absorption spectrometry. Liver samples were collected after experiments and stained by immunohistochemistry of ?-SMA. Moreover ?-SMAmRNA expression in liver tissue was detected by RT-PCR technology. Results Ganle keli could significantly reduce NO, NOS and iNOS contents, effectively prevent the expression of ?-SMA, and significantly reduce ?-SMA mRNA in liver fibrosis rats. Conclusion Ganle keli can effectively inhibit the expression of ?-SMA in CCl4-induced fibrotic liver in rats.
