Determination of Emodin in Niuhuang Anti-inflammatory Tablets by HPLC
- VernacularTitle:高效液相色谱法测定牛黄消炎片中大黄素的含量
- Author:
Fei MA
;
Zhiwei YANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Niuhuang anti-inflammatory tablets;
emodin;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2008;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of emodin in Niuhuang anti-inflammatory tablets. Method Thermo-Hypersil C18 (250 mm?4.6 mm, 5 ?m) was used, mobile phase was methanol-0.1% phosphoric acid (70∶30), with flow rate of 1.0 mL/min, detection wavelength of 254 nm, and column temperature was 40 ℃. Results There was a good linear relationship of emodin at the range of 0.020 11～0.402 2 ?g. Conclusion The method is simple, accurate, with better separation efficiency, wide linear range and high sensitivity. It can be used for quality control of the preparation.
