Identification of clematis raw drugs by HPLC fingerprint
- VernacularTitle:威灵仙类药材HPLC指纹图谱鉴别研究
- Author:
Xiangpei WANG
;
Jie HUANG
;
Fengyun JIN
;
Yichun SUN
;
Hongmei WU
;
Shina XU
- Publication Type:Journal Article
- Keywords:
Clematis umcinata Champ;
clematis;
HPLC;
fingerprint;
identification
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish HPLC fingerprint for the root and rhizome of Clematis uncinata Champ and to compare the differences of clematis and Clematis uncinata in fingerprint. METHODS:Based on 10 batches of Clematis unciuata Champ,its chromatographic seperation was performed on Diamonsil C18 (250 mm ? 4. 6 mm,5 ?m)with a mobile phase consisting of acetonitrile -0.05% phosphoric acid,gradient eluent,at the flow rate of 0. 8 mL/min. The UV detection was set at 210 nm. RESULTS:The mutual mode to HPLC-UV fingerprints was set up,and the 23 mutual peaks were indicated. The similarities were compared among Cleuatis uncinata Champ and substitutes collected from different sources there were apparent difference in fingerprint. CONCLUSION:The method is stable and reliable with a good reproducibility and provides a reference standard for identifying medicinal clematis.
