One step separation and preparation of senkyunolide A and Z-ligustilide in Ligusticum chuanxiong Hort by high speed counter current chromatography
- VernacularTitle:应用高速逆流一次性分离川芎中的蛇床内酯A和Z-蒿本内酯
- Author:
Wen LIU
;
Pingli WU
;
Chao ZHUO
;
Jiquan ZHANG
;
Pingniang SHEN
- Publication Type:Journal Article
- Keywords:
high speed counter current chromatography;
Ligusticum chuanxiong Hort;
senkyunolide A;
Z-ligustilide
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To research how to separate the active component in Ligusticum chuanxiong Hort by high speed counter current chromatography. METHODS: Senkyunolide A and Z-ligustilide,the main components of volatile oil in Ligusticum chuanxiong Hort were one step separated by high speed counter current chromatography. n-hexane-ethyl acetate-ethanol-water,1 ∶ 1 ∶ 1 ∶ 1(v/v),was used as the solvent system for HSCCC. Top phase and bottom phase were respectively used as static phase and mobile phase. Optimum speed and flow rate were 900 r/min and 1. 2 mL/min respectively. RESULTS: Collected fractions were analyzed by HPLC and identified by EI-MS and 1HNMR. Purity could reach more than 95% . CONCLUSION: Lactone is fit to be separated and prepared by high speed counter current chromatography with good resolution and high purity. We find a fast and efficient way to separate these.
