Effect of ?-elemene on proliferation of human multiple myeloma cells
- VernacularTitle:?-榄香烯抑制人骨髓瘤细胞增殖
- Author:
Hao CHEN
;
Liang SHI
;
Suyun WANG
;
Jingci YANG
;
Ling PAN
- Publication Type:Journal Article
- Keywords:
?-elemene;
multiple myeloma;
proliferation;
cell cycle;
apoptosis
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of ?-elemene,extracted from curcuma wenyujin,on proliferation, cell cycle and apoptosis of human multiple myeloma RPMI-8226 cells. METHODS: The effect of ?-elemene on the growth of human multiple myeloma RPMI-8226 cells was studied through MTT assay,cell cycle and apoptosis was studied by combined Annexin-V protein iodide staining,The morphological changes was studied by combining Hoechst33342-PI staining. RESULTS: ?-elemene inhibited the proliferation of RPMI-8226 cells in a time-and dose- dependent manner. Compared with the control cells,the proportions of the RPMI-8226 cells in the G0 /G1 phase rose,and the proportions of the RPMI-8226 cells in the S and G2 /M phases fell decreased. RPMI-8226 cells treated with ?-elemene for 48 h induced apoptosis in a dose-dependent manner. CONCLUSION: ?-elemene is able to inhibit the proliferation of RPMI-8226 cells by arresting the cell cycle and inducing the cell apoptosis.
