Effect of Huoxuetongluo Decoction on Expression of CD54 and Polymorphonuclear Adhesion during Anoxia/Reoxygenation Injury in Human Umbilical Vein Endothelial Cells
- VernacularTitle:活血通络汤对人脐静脉内皮细胞缺氧/复氧损伤CD54表达及中性粒细胞粘附率的影响
- Author:
Yong MA
;
Yunshen ZHANG
;
Jinfei CHEN
- Publication Type:Journal Article
- Keywords:
Huoxuetongluo Decoction;
anoxia/reoxygenation;
vascular endothelial cell;
CD54;
polymorphonuclear;
adhesion
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Huoxuetongluo Decoction on expression of CD54 and polymorphonuclear adhesion during human umbilical vein endothelial cells(HUVEC) anoxia/reoxygenation injury,and explore its possible mechanisms.Methods Rabbits serum containing Huoxuetongluo Decoction was prepared.Resuscitate and culture HUVEC,then establish the anoxia/reoxygenation injury model of HUVEC.The model cells were divided into five groups:normal control,model group and group of high,medium,low dose of Huoxuetongluo Decoction.After dealt seperately,the morphologic change of cells were observed through the microscope,the expression of CD54 and the polymorphonuclear adhesion were determined.Results The group of low,medium and high dose of Huoxuetongluo Decoction inhibited the expression of CD54 and decreased the adhesion between polymorphonuclear and HUVEC,the effects were strengthened with increasing the dose of Huoxuetongluo Decoction.The difference between group of medium,high dose of Huoxuetongluo Decoction and model group had statistical significance(P
