Effect of Tetramethyl Pyrazine on Infarct Areas and Expressions of ED1,IL-1?,TNF-? after Focal Cerebral Ischemia Reperfusion in Rats
- VernacularTitle:川芎嗪对大鼠脑缺血再灌注后梗死面积及ED1、IL-1?和TNF-?表达的影响
- Author:
Yanchun MA
;
Yao CHEN
- Publication Type:Journal Article
- Keywords:
Tetramethyl Pyrazine;
cerebral ischemia reperfusion;
ED1;
interleukin-1?;
tumor necrosis factor-?;
rat
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Tetramethyl Pyrazine(TMP) for treating cerebral infarct by observing infarct areas,and to research its function mechanism by observing the changes of the expressions of ED1,TNF-? and IL-1? in the infarction region after focal cerebral ischemia reperfusion injury.Methods Sixty SD male rats in healthy condition were randomly and averagely divided into normal group,model group and TMP high,middle and low dose group.The cerebral infarct animal model was reproduced by modified thread-tie method.TMP high dose group was administered TMP 140 mg/kg,middle dose group was 120 mg/kg and low dose group was 100 mg/kg by intravenous.Then all the rats were killed.Thirty rats were taken to evaluate whether TMP can decrease the ratio of cerebral infarction areas in the TTC-reacting and formalin fixation brain tissue slices taken from the above-mentioned killed rats,with Pathology Image Analysis System.The masculine of ED1,IL-1? and TNF-? in the immuno-reacting cells in cerebral infarction brain tissue slice taken from other thirty killed rats were observed.Results TMP groups decreased the ratio of cerebral infarction areas,and also decreased ED1,TNF-? and IL-1? in immuno-reacting cell.Conclusion TMP can decrease the formation of cerebral infarction caused by cerebral ischemia reperfusion and its effect is possibly related to the activation of microglia,as well as the immuno-positive expressions of TNF-? and IL-1?.
