The expression of surfactant protein genes hyperoxia-induced neonatal rat lung injury
- VernacularTitle:高氧诱导肺损伤新生鼠肺表面活性蛋白基因表达的研究
- Author:
Jiguo SONG
- Publication Type:Journal Article
- Keywords:
Hyperoxia;
Surfactant protein;
Lung injury;
Newbornrats
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of hyperoxiaon the changes of pulmonary histology and surfactant protein gene expression.Methods:SD newborns which less than 6 h old were randomly divided into 2 groups:normal air control group and hyperoxia group.The lungs of rats were removed at the time points 1,4,7,14 d,histopathological changes of the lung tissues were examined by HE staining and the lung factor were calculated(lung weight/body weight ?100%);the expression of all surfactant protein genes were detected by semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR).Results:To compare with corresponding air control group,in hyperoxia group,the body weight is significantly deceased after 14 days of hyperoxia exposure(P0.05),and began to significantly decreased at 14 d(P
