Effect of allitride injection and ischemic preconditioning on renal ischemia/reperfusion injury in rats
- VernacularTitle:大蒜素及缺血预处理对大鼠肾缺血再灌注损伤的影响及机制研究
- Author:
Jianwei WU
- Publication Type:Journal Article
- Keywords:
Ischemic precondiontionging;
Kidney ischemia/reperfusion;
Allimin;
Intercellular-adhesion molecule-1(ICAM-1);
Malon aldehyd(eMDA);
Superoxide dismustase(SOD)
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of allitride injection preconditioning and ischemic preconditioning on renal ischemia/ reperfusion(I/R)injury in rats.Methods:Rat's I/R injury model was established.A total of 75 rats were randomized into five groups(n=15,in each);control group(CON),I/R control group(IR),ischemic preconditioninggroup(IPC),allitride preconditioninggroup(APC),allitride preconditioning and ischemic preconditioning group(AIPC).Kidney tissue and blood specimen were collected from all groups 24 hours after operation.The changes of the renal pathological morphology were observed by hematoxylin-eosine(HE)staining.The levels of serum creatinine(Cr)and urea nitrogen(BUN) were determined.The levels of malonaldehyde(MDA)were determined by thibabituric acid(TBA).The levels of superoxide dismutase(SOD)were determined by xanthine oxidase.Intercellular adhesion molecule-1(ICAM-1)expression in kidney was tested by immunohistoehemistry.Results:IPC or APC group had less necrosis in HE staining than IR group.BUN,Cr,MDA contents in IPC or APC group are less than that in IR group(P
