Protection of adenosine A1 receptor agonist delayed preconditioning for myocardial ischemia reperfusion injury in rabbits
- VernacularTitle:腺苷A1受体激动剂延迟预处理对兔心肌缺血再灌注损伤的保护作用
- Author:
Ke RAN
- Publication Type:Journal Article
- Keywords:
Adenosine A1 receptor agonis(tCCPA);
Delayed preconditioning;
Myocardium;
Ischemia reperfusion
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protection mechanism of adenosine A1 receptor agonist(2-chloro-N6-cyclopentyladenosine, CCPA)delayed preconditioning for rabbit myocardium during ischemia reperfusion.Methods:Thirty New Zealand male white rabbits were randomly assigned to Sham group,I/R group and CCPA group,with ten in each.Group CCPA was given CCPA 0.1 mg/kg before myocardial ischemia.Twenty-four hours later the I/R and CCPA underwent 40 min of coronary occlusion followed by 2 h of reperfusion.Blood samples were taken from arterial line at 20 min before occlusion(T)1,20 min after occlusion(T2),40 min after occlusion(T)3,1 h after reperfusion(T)4and 2 h after reperfusion(T)5for determination of the plasma levels of IL-10.At the end of the reperfusion,infarct size(IS)and area at risk(AAR)were defined by Evans blue and TTC staining.The heart was harvested and levels of the HSP70 were determined by Western blot analysis,and ultrastructures were observed under the electron microscopy.Results:The IL-10 and HSP70 levels of group CCPA was higher than those of group I/R.The CCPA(P
