The separation and determination of ketoprofen enantiomers in plasma by chiral column
- VernacularTitle:酮洛芬对映体的分离、分析方法的研究及其应用
- Author:
Xiaomei LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Ketoprofen;
Enantiomers;
Chiral separation
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The separation effect for the enantiomers of ketoprofen on four diferent chiral columns was investigated and established a sensitive and stereospecific HPLC separation and determination method for ketoprofen enantiomers in animal plasma.Methods:The enantiomers of ketoprofen was resolved in the normal phase mode by using n-hexane∶isopropanol∶TFA(80∶20∶0.02)as mobile phase with UV detector at a wavelength of 254 nm.The flow rate was 1.0 ml/min.Results:Linear calibration curves were obtained in the range 0.1～10?g/ml for R-(+)-ketoprofen(r=0.999 8)and S-(+)-ketoprofen(r=0.999 6)in plasma.The detection limit(S/N=3) was 2 ng for each enantiomer.Conclusion:This method is simple,fast and can be used to detect optical purity of S-(+)-ketoprofen and monitor pharmacokinetic studies of ketoprofen enantiomers.
