Selection of formulation for IL-2 targeting liposome and determination of drug contents
- VernacularTitle:IL-2靶向脂质体的处方筛选及药物含量的测定
- Author:
Meichun WU
- Publication Type:Journal Article
- Keywords:
5-FUR prodrug;
Orthogonal design;
UVspectrophotometry;
Content determination
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Selection ofthe optimal formulation for IL-2 targeting liposome and determination ofcontent for 5-FUR.Methods:orthogonal test method was used to selection the optimal formulation,UV spectrophotometry was used to determine the 5-FUR prodrug content,and the detection wavelength was at 267 nm.Results:The optimal formulation showed Drug/EPC 1∶10,EPC/Ch 3∶1.The absorbency and concentration of 5-FUR prodrug had good linearity in the range of 2.0～20.0?g/mL.The regression equation was y=16.357x+0.007,r= 0.999 7(n=3),The 5-FUR prodrug content was 18.84～18.91?g/ml.The average recovery rate was 97.09%,and RSD=2.05%(n=3).Conclusion:Formulation was designed reasonablely.The method is simple,stable and reliable,which can be used to determine the 5-FUR prodrug in IL-2 targeting liposome.
