Genetic polymorphisms of four STR loci on chromosome X and their forensic application in Chongqing han population
- VernacularTitle:重庆地区汉族人群X染色体4个STR基因座的遗传多态性及其法医学意义
- Author:
Hong LI
- Publication Type:Journal Article
- Keywords:
Chongqing;
Han nationality;
X-STR;
Genetic polymorphism;
Forensic medicine
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate genetic polymorphism of four X chromosome short tandem repeats(X-STR)loci(DXS7132、 DXS6789,HPRTB,DXS8378)in Chongqing Han population.Methods:EDTA-blood samples were collected from the unrelated individuals in Chongqing.After being extracted with Chelex-100 method,the DNA samples were amplified by PCR technique.The PCR products were analyzed by PAGE,genotyped with silver stain method.Hardy-Weinberg equilibrium was tested,and then used direct counting method to calculate allele and genotype frequency of these four loci.Results:The polymorphisms of all four STR loci were obtained from 120 unrelated females and 91 unrelated males from Chinese Han group.?2 tests on the genotype frequencies in female reveal that genotype frequencies in females did not depart from Hardy—Weinberg equilibrium.Conclusion:It was suggested that DXS7132,DXS6789,HPRTB,DXS8378 locus are suitable for individual identification and paternity testing,and we provide new selectable STR markers for chromosome X haplotype in forensic application.
